Recombinant proteins are widely used in the fields of medicine and biotechnology research. You may not be aware of it but recombinant proteins are extensively being used in pharmaceutical and medical testing laboratories, doctors' and veterinarians' offices and biological research laboratories, to name a few. Practical applications of this technology are also found in the fields of agriculture, food processing, industry and bioengineering.With the extensive application of this technology, are you starting to wonder how recombinant proteins are made?
Recombinant Proteins – How Are They Made?
The most common method by which recombinant proteins are mass produced for laboratory and industrial purposes is called molecular cloning. There are three basic requirementsincreating recombinant proteins - the coding sequence for the heterologous protein, the cloning vector (usually a plasmid or virus) into which the protein of interest will be cloned and a suitable host (can be a bacteria, yeast, insect or mammalian cell) that will express the protein of interest.
